Figure 7
From: A novel HIV-1-encoded microRNA enhances its viral replication by targeting the TATA box region
Effect of small RNAs targeting HIV-1 TATA box upon HIV-1 production from resting CD4+T cells isolated from HIV-1–infected patients on suppressive HAART. (A) SiRNAs against HIV-1 TATA box were synthesized with the illustrated sequences. The TATA box motif was indicated with a box. (B) The miR-H3 miRNA mimic or siRNA was co-transfected with pNL4-3-deltaE-EGFP into HEK293T cells. The productions of P24 in the supernatant were determined with P24 ELISA at 48 hrs post-transfection. (C) Resting CD4+ T cells isolated from HIV-1-infected patients on suppressive HAART were transfected with control siRNA or si-HIV-TATAs. After 72 hrs, HIV-1 virions in the supernatants were collected and viral RNAs were isolated and amplified by real-time q RT-PCR with primer SK38 and SK39. The cutoff for virion-associated RNA is 500 copies per ml. P-values were calculated using the two tailed unpaired Student’s t-test with equal variances, n = 3. *p < 0.05, **p < 0.01.