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Figure 7 | Retrovirology

Figure 7

From: Human protein Staufen-2 promotes HIV-1 proliferation by positively regulating RNA export activity of viral protein Rev

Figure 7

Rev-hStau-2 interactions are essential for hStau-2 dependent promotion of Rev activity. Viral RRE-RNA levels were measured in the cytoplasmic fraction by absolute qRT-PCR for either hStau-2 or hStau-2Mut over-expressing cells. A) hStau-2Mut did not promote RRE-RNA export activity of Rev: Viral RRE-RNA levels were measured in the cytoplasmic fraction by absolute qRT-PCR for hStau-2, hStau-2Mut over-expressing cells and the control cells. Concentrations of the plasmid hStau-2 or hStau-2-Mut was 0.1ug and pNL4-3 was 40 ng. B) Viral p24 levels were scored by p24 ELISA. Concentration of the plasmid hStau-2 or hStau-2-Mut was 0.5ug and pNL4-3 was 40 ng. hStau-2Mut did not increase HIV-1 production.

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Retrovirology

ISSN: 1742-4690

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