Figure 1

HIV-1 envelope expression profiles on infected CD4+ T cells. CEM.NKR (A and B) or activated primary CD4+ T cells (C and D) were infected with CCR5-tropic NL4.3.ADA.IRES.GFP wild-type (WT) virus or derivatives lacking Vpu (U-), Nef (N-) or both (N-U-) as detailed in Methods. Infected cells were analyzed by flow cytometry for Env expression using anti-Env A32 and 2G12 mAbs. (A-D) The left panels depict the extent of Env staining shown as geo-mean fluorescence intensity (MFI) on gated GFP⁺ cells from a representative infection. The right panels summarize the results of (A and B) seven experiments with each dot representing an analysis, and of (C and D) data obtained with primary CD4+ T cells from four donors. Shown are average fold increase (+/- SEM) in Env staining relative to WT virus-infected cells. Fold increase was determined as the ratio of MFIs of GFP⁺ cells infected with different mutants over that for the WT virus. Statistical analysis of data was done using paired Student’s t-tests.