Figure 4

Variable effect of CsA on transduction of different cell lines by HIV-1 bearing the A92E CA mutant. A panel of 27 cell lines and human CD4+ T cells were treated with 5 μM CsA or DMSO as control and challenged with an HIV-1-GFP reporter vector bearing the A92E CA mutation. 72 hrs post-transduction the percent GFP⁺ cells was determined by flow cytometry, as an indicator for infectivity. The ratio of A92E mutant virus infectivity in CsA vs DMSO treated cells is shown. Data represent one of at least two independent experiments. Error bars represent ± SEM.