Figure 11

Anti-Gag response to a live vector boost. (A) Group 4 macaques were primed with the rubella vaccine strain at 0 and 5 weeks. They were boosted at week 57 with rubella vectors expressing BC-sGag2-E2TM and HIV MPER. (B) Group 1 macaques were primed three times with DNA vaccine, followed by a live BC-sGag2 vector at week 25. They were boosted at week 57 with novel vectors expressing an MPER–BC-sGag2-E2TM fusion protein and the entire p27 Gag protein. Animals primed with empty rubella vaccine had no antibodies to Gag and failed to respond to the BC-sGag2 boost. Animals primed with gag DNA and rubella expressing BC-sGag2 had persistent antibodies at week 57 (open symbols), and they responded strongly to the boost (solid symbols). This indicates that DNA/rubella priming induced memory B cells, which were recalled by the boost.