Figure 2

Associations between CCR5 usage and usage of CCR3, FPRL1 and CCR8 by R5 C-HIV Envs. Luciferase reporter viruses pseudotyped with each of the C-HIV R5 Envs (n = 294) were used to infect NP2-CD4 cells expressing CCR5, FPRL1, CCR3 or CCR8, and the levels of HIV-1 entry were determined as described in the Methods. Comparative entry levels for each of the individual Envs are shown in Additional file 1: Table S1. (A), scatterplots of all 294 R5 Envs comparing the levels of HIV-1 entry via CCR5 to that of CCR3 (left), FPRL1 (middle), and CCR8 (right). (B), these data were then stratified according to whether the Envs were cloned from the “enrolment” (top row), “intermediate” (middle row) of “final” (bottom row) plasma samples from the longitudinal cohort [8]. Each dot represents the mean value of triplicate experiments. Statistical analysis of these data is shown in Table 1.