Figure 4

Splenic peripheral B cell population in HIV Tg26 mice. Flow analysis was performed in splenocytes from wild type mouse FVBN (WT), Tg26 without skin lesions (Tg no S), Tg26 at early stage of splenomegaly (Tg pre-L) and a Tg at advanced stage of splenomegaly (Tg L). Splenic B cells comprised two major populations at side/forward scattered-high (SSC/FSC_hi), corresponding to non-activated and activated populations (Figure 3 shows only the activated population). Starting from the left side, flow cytometry analysis of splenic B cell population in HIV Tg26 mice displayed: (A) decrease of T versus B cell ratio, as shown by CD3 and CD19 staining; (B) decrease of immature (B220⁺IgD^-IgM⁺) and mature (B220⁺IgD⁺IgM^-) B cells; (C) decrease in follicular (B220⁺CD21^-CD23⁺) and marginal zone (B220⁺CD21⁺CD23^-) B cells; (D) no change in IgG1-expressing B cells (B220⁺IgG1⁺); (E) reduction in plasma cells (CD19⁺CD138⁺); (F) decrease in B1B cells (B220⁺CD5⁺); (G) increase of precursors B cells (B220⁺CD43⁺CD93⁺); (H) increase of B220⁺Sca⁺ B cells; undetectable c-kit and (I) increase of B220⁺CD34⁺ B cells.