Figure 4

Characterization of the nature of the LTR-LTR junction. MT4 cells were infected with the VSV-G-pseudotyped NLENG1-ES-IRES WT (+/- RAL 500 nM) or NLENG1-ES-IRES D116N viruses. Each value corresponds to an average of five to six independent experiments and confidence intervals analysis are shown for a p value <0.05. (A) 2-LTRc as a percentage of total HIV-1 DNA 24 h post-infection (black column, global 2-LTRc and grey column, 2-LTRc harboring a perfect LTR-LTR junction). The fold increase in the amount of 2-LTRc (between integration defective conditions (WT + RAL and D116N) and the WT) is indicated at the top of the columns. (B) 2-LTRc harboring a perfect palindromic sequence as a percentage of total 2-LTRc in each condition of infection (WT, WT + RAL and D116N). (C) Standard curves with p2-LTR using primers and probes for quantification of 2-LTRc harboring a perfect LTR-LTR junction (left panel) and for quantification of total 2-LTRc (right panel).