Figure 6

Impact of variants on the ability of tetherin to activate NF-κB. (A) Activation of NF-κB-dependent firefly luciferase reporter gene expression in 293T cells transiently cotransfected with tetherin, NF-κB-dependent (three NF-κB binding sites) or -independent firefly luciferase constructs and a reporter plasmid expressing gaussia luciferase under the control of a minimal promoter. The results show mean values ± SD of three independent transfections. (B) Equal amounts of vectors expressing wild-type and R19H tetherin were transfected to mimic heterozygosity. Mean values ± SD of four independent experiments in triplicates are shown.