Figure 3

Bet and Bel2ORF bind to feA3Z2b. Bet and Bet N-terminal deletion mutants were expressed in 293T cells and pulled down either with GST or GST-feA3Z2b. Immunoblot analysis was performed with Bet-specific serum (upper panel) and an SV40 tag-specific antibody to detect the GST and GST-feA3Z2b fusion proteins (lower panel). Cell lysates (CL) of transfected cells were analyzed in parallel to confirm proper expression of wt and mutant Bet proteins. In addition to wt Bet, Bel2ORF was pulled down with GST-feA3Z2b. Due to high protein levels, some bands have ‘shades’ (lower panel). Symbols below the image indicate the presence (+) or absence (-) of GST or GST-feA3.