Figure 5
Acetylation does not prevent interaction of the Tax with PDZ-binding protein hDLG. (A) 293T cells transfected with vectors expressing HA-hDLG and WT Tax or mutant K346R were subjected to anti-Tax immunoprecipitation (IP Tax) and analyzed by Western Blotting with anti-HA antibody. Total lysates were analyzed by Western Blotting with anti-Tax and anti-HA antibodies. (B) 293T cells expressing WT Tax or the K346R mutant alone or with HA-hDLG were analyzed by immunofluorescence staining and confocal microscopy with anti-Tax IgG2a mAb and anti-HA rabbit polyclonal antibody for the detection of HA-DLG.