Figure 4

Activation of the NF-κB and ATF/CREB pathways in Rat-1 cells expressing wild type Tax. (A) Rat-1 cells transducted with either the control lentiviral vector (−) or with the lentiviral vector expressing wild type Tax (Tax) were transfected with 0.5 μg of κB-luc (grey bars) or 0.5 μg HTLV-1 LTR-luc (white bars) reporter constructs and 0.5 μg of pRL-TK-luc. The results are presented as fold increase of luciferase activities in Rat-1 cells expressing Tax relatively to control Rat-1 cells. The values are averages of three independent experiments, with standard deviations. The numbers above the bars represent basal luciferase activities in control Rat-1 cells. (B) Control Rat-1 cells (−) or Rat-1 cells expressing Tax (Tax) were analyzed by triple immunofluorescence staining with anti-Tax IgG2a mAb, anti-RelA rabbit polyclonal antibody and anti-p300 IgG1 mAb followed by confocal microscopy.