Figure 7

E45A HIV-1 cores allow vRNA and NC staining more readily than WT HIV-1 cores. (A) Equal amounts of p24 of EU-labeled WT HIV-1 and E45A HIV-1 were treated with cell extraction buffer for 15 minutes, fixed onto slides, and then stained for EU (green) and NC (red). Particles were visualized by fluorescent confocal microscopy. (B) RNA staining of WT HIV-1 and E45A HIV-1 was counted alone and when co-localized with NC. The percentage of co-localized events for at least four fields was calculated. Data represent the mean ± SEM of at least 4 fields. (C) Equal amounts of p24 of EU-labeled WT HIV-1 and E45A HIV-1 also containing MS2-GFP (green) were treated with cell extraction buffer for 15 minutes, fixed, and stained for EU (red). Particles were visualized by fluorescent confocal microscopy and shown for each individual color and with both colors (merge).