Figure 4

Viral elimination is constant over a range of inoculum sizes. (A) Representative FACS plots showing the titration of gag positivity from a viral inoculum size of 1000 ng to a viral inoculum size of 62.5 ng per one million cells. (B) The average percent infection as a result of viral titration was calculated over a range of viral inoculum sizes in a subset of our ES cohort (n=6; top panel). Unstimulated CD8⁺ T cells were added to either untreated (Blue squares) or 10 μM EFV treated ( Red squares) CD4⁺ T cells targets at a 1:1 effector to target ratio and the level of elimination was calculated 72 hours after infection (bottom panel). Statistically significant differences in elimination between the viral inoculum size for the untreated CD4⁺ T cell target group is indicated (blue bracket and asterisks). Differences in elimination between the EFV treated and untreated treatment groups are indicated (black asterisks).