Figure 1

Early Gag positivity in spinoculated cells is CD4 and co-receptor dependant. CD4⁺ T cells were either untreated or treated with anti-CD4 antibody, MVC, ADM, or T20 for the duration of spinoculation. Percent Gag⁺ cells were analyzed immediately after spinoculation with R5 (A) or X4 (B) pseuotype virus. (C) X4 virus infection was analyzed at 0, 6 and 18 hours post spinoculation after treatment with anti-CD4 antibody, MVC, ADM, or T20 for the duration of the culture period. (D) Comparision of the percent infection 24 hours after spinoculation with and without T20 treatment (n=3). Standard error of the mean are indicated by black bars.