Figure 1

Inhibition of WT and K65R RT-catalyzed DNA synthesis by EFdA-TP. T_d31/P_d18-P0 was incubated with WT or K65R HIV-1 RT for 50 minutes in the presence of 1 μM dNTPs, MgCl₂ and increasing concentrations of EFdA-TP (0–1,500 nM). The experiment was carried out in the (A) absence or (B) presence of 3.5 mM ATP. The template sequence is shown next to the gels and the numbers indicate the points of EFdA-TP incorporation (+1, +6 and +10).