Figure 5
From: Critical roles for Akt kinase in controlling HIV envelope-mediated depletion of CD4 T cells
Env-dependent CD4-mediated activation of Akt increases CXCR5/PD-1 expression and Fas-dependent cell death. (A) Purified tonsil CD4 T cells were treated with or without BaL-gp120 for 3 days. In some cases, neutralizing anti-Fas antibody ZB4 (1 μg/ml), sCD4 (10 μg/ml) or Maraviroc (1 μM) was added to the T cell cultures. Expression of CXCR5 and PD-1 were monitored every day by flow cytometry. (B, C) CXCR5hiPD-1hi cells were purified by cell sorting and cultured in the absence or presence of BaL-gp120 for 3 days. Expression of CXCR5, PD-1 (B), or Fas (C) was detected by flow cytometry. (D) CXCR5-PD-1- cells were purified by cell sorting and cultured in the absence or presence of BaL-gp120 for 3 days. In some cases, sCD4 (10 μg/ml), Maraviroc (1 μM), or specific inhibitors (2 μM) for Akt, Erk or p38, were added to cell cultures. Expression of CXCR5 and PD-1 were detected by flow cytometry. Data are representative of three independent experiments.