Profiles of resistance to MVC and cross-resistance to other CCR5 antagonists. Luciferase reporter viruses pseudotyped with Envs from subject 17 or subject 24 were used to infect NP2-CD4/CCR5 (A, left panels) or PBMC (A, right panels) in the presence of increasing concentrations of MVC. The same virus preparations were used to infect NP2-CD4/CCR5 cells in the presence of increasing concentrations of VVC or TAK-779 (B). Virus inhibition curves were constructed as described in the Methods. The data points represent the mean and standard error of the mean from quadruplicate wells, and are the results from 5 (MVC) or 3 (VVC and TAK-779) independent experiments. The independent PBMC experiments were performed in cells obtained from different donors. Inhibition curves were constructed using Prism, version 4.0c (GraphPad).