Figure 1

Vpx poorly rescues HIV-1 transduction in IFN-α-primed monocytes/MDMs despite SAMHD1 degradation. (A) Monocytes from two donors were either mock treated, or treated with IFN-α or IFN-γ (10000 U/ml). After 24 h, cells were incubated with empty or Vpx-containing VLP (X- and X+, respectively) for 2 h, and transduced with a VSV-G-pseudotyped GFP-encoding HIV-1 virus for 2 h at a multiplicity of infection (MOI) of 1. Three days post-transduction, the percentage of infected cells (% GFP + cells) was measured by flow cytometry. ND: not detected. (B) Same experimental procedure as in (A) performed with MDMs derived from the monocytes of the 2 donors presented in (A). (C) Same experimental procedure as in (B) performed in MDMs of 2 different donors, except that the concentration of IFN-α and IFN-γ has been reduced to 1000 U/ml. (D) SAMHD1 as well as APOBEC3A (A3A) protein levels were assessed by Western blot analysis from the cells in (C) two days post transduction (one representative Western blot is shown). (E): Western blot analysis of SAMHD1 and A3A expression in MDMs from two donors challenged or not (Ctrl) for the indicated periods with 1000 U/ml of IFN-α and IFN-γ.