Figure 2
From: Viral fitness cost prevents HIV-1 from evading dolutegravir drug pressure
Effects of the H51Y and R263K mutations on HIV infectivity and replicative fitness. (A) pNL4.3IN(WT), pNL4.3IN(H51Y), pNL4.3IN(R263K), and pNL4.3IN(H51Y/R263K) infectivity were measured by quantifying luciferase activity in relative luminescent units (RLU) produced by TZM-bl cells infected with increasing concentrations of virus (in ng of p24 antigen). Lines are fits. (B) Reverse transcriptase (RT) activity was measured as counts per minute (cpm) per day in the culture fluids of PM1 cells infected with pNL4.3IN(WT), pNL4.3IN(H51Y), pNL4.3IN(R263K), or pNL4.3IN(H51Y/R263K) virus. Error bars indicate ± s.e.m.