Figure 7

HBZ overcame the C/EBPα-mediated growth suppression. (A) Mouse CD4⁺CD25^- T cells were transduced with pGCDNsamI/GFP vector encoding C/EBPα, or with empty vector. At three days after infection, cell proliferation was analyzed by flow cytometry. (B) Mouse CD4⁺CD25^- T cells were transduced with pGCDNsamI/NGFR vector encoding HBZ together with pGCDNsamI/GFP-C/EBPα. Cells were stained with specific antibody at the time points indicated, and cell growth was detected by flow cytometry. Representative data from three independent experiments are shown. (C) HBZ modulated the expression of selected C/EBPα target genes. Total RNA was extracted from samples from the experiment of Figure 7B. The level of MYC, FLIP, IFN-γ, BCL2, DHFR, E2F1, PER2, CDKN1A, IL6, IL4, PCNA, CDK2, β-actin, CEBPA, and HBZ mRNA were analyzed by semiquantitative RT-PCR. (D) Transcriptional changes of selected C/EBPα target genes in CD4⁺ thymocytes from HBZ transgenic mice. After stimulating the cells with PMA plus ionomycin, the levels of MYC, FLIP, E2F1, PER2, IL6, PCNA, β-actin, CEBPA, and HBZ mRNA were analyzed by semiquantitative RT-PCR.