Figure 6

Assessment of IFN and CD107a expression by CD7⁺CD56⁺CD16⁺and CD7⁺CD56^negCD16⁺NK cells following in vitro stimulation. A-B IFNγ expression was compared between CD7⁺CD56⁺CD16⁺ and CD7⁺CD56^negCD16⁺ NK cells in (A) healthy and (B) chronic HIV-1-infected donors following incubation in media alone, IL-12 plus IL-18, or K562 target cells. C-D, CD107a expression was compared between CD7⁺CD56⁺CD16⁺ and CD7⁺CD56^negCD16⁺ NK cells in (C) healthy and (D) chronic HIV-1-infected donors following incubation in media alone or K562 target cells. A total of 10 healthy and 19 chronic HIV-1-infected donors were assessed. A minimum of 200 cells and 70 cells were analyzed for IFNγ and CD107 expression in CD7⁺CD56⁺CD16⁺ and CD7⁺CD56^negCD16⁺ NK cells, respectively, of healthy donors. A minimum of 550 cells and 100 cells were analyzed for IFNγ and CD107 expression in CD7⁺CD56⁺CD16⁺ and CD7⁺CD56^negCD16⁺ NK cells, respectively, of HIV-1-infected donors. The median and 25th and 75th percentile are indicated on each graph. ( = CD7⁺CD56⁺CD16⁺ NK cells; = CD7⁺CD56^negCD16⁺ NK cells).