Figure 4
Soluble Siglec-7 facilitates HIV-1 entry in CD4posT cells and MDMs. A-B, Histogram bar graph showing the copies/ml of HIV-RNA transcripts in the CD4pos primary T cell lysates infected with IIIB (A) or BaL (B) viral strains either pre-incubated or not with either NKp44 or Siglec-7 fusion proteins. C, Histogram flow cytometric graph from a representative example showing the surface expression of Siglec-7 on monocytes (black line) and on MDMs (grey line) compared to isotype control (light gray line). D, Histogram bar graph showing the copies/ml of HIV-RNA transcripts in the MDM cell lysates infected with BaL viral strain either in the absence or in the presence of a blocking polyclonal antibody anti-Siglec-7. The related isotype control is included in the graph. Data are representative of 4 independent experiments (mean ± SD).