Figure 2

Susceptibility of PBMCs derived from macaques assigned to the in vivo study to SIVmac239 infection and to SP- or SEVI-mediated enhancement of infection. (A, B) Effect of SEVI (A) and SP (B) on SIVmac239 infection of PBMCs derived from the 18 animals assigned to the in vivo study. PHA/IL-2 stimulated cells were infected with SIVmac239 encoding a luciferase reporter gene [22]. Numbers above the bars indicate n-fold infectivity enhancement observed in the presence of SP or SEVI compared to those measured in their absence. RLU/s: relative light units per second. (C, D) PBMCs were grouped based on their belonging to the control (co), SEVI or SP groups in the subsequent in vivo study. The box plots in C show the mean infection rates and 25th and 75th percentiles measured in the absence of SP and SEVI. The box plots in (D) show the n-fold enhancement of SIVmac239 infection of PBMCs derived from the three groups of macaques in the presence of SEVI (10 μg/ml) or SP (10% v/v).