Figure 7

Binding to IN-CCD wt, T174I mutant, treatment during production of wt and T174I mutant viruses. (A) Binding kinetics of Mut101 to IN-CCD wt. Serial dilutions of Mut101 (between 9.8 nM and 5 μM) were injected on immobilized GST-Flag-CCD wt. (B) Binding kinetics of Mut101 to IN-CCD T174I. Serial dilutions of Mut101 (between 1.3 μM and 5.0 μM) were injected on immobilized GST-Flag-CCD T174I. (C) Diagram of the experimental setup to study the infectivity of NL4-3 wt and IN T174I virions produced after transfection of 293 T cells. The indicated compounds were added during virus production for 48 h. Supernatants were tested for virus production by p24 assay, and for virus infectivity. (D) Infectivity of wt NL4-3 (blue bars) and IN T174I NL4-3 mutant (red bars) virions harvested from 293 T transfected cells after treatment with the indicated compounds and infection of MT4 cells by cytopathic assay using CellTiter-Glo®.