Figure 5

Time of addition experiment and qPCR analysis. (A) Time-of-addition (TOA) experiment in single-round infection assay of MT4 cells infected with VSV-G-pseudotyped HIV-1 Δenv-Luc NL4-3. Each compound (Mut101, Nevirapine (NVP) or Raltegravir (RAL)) was added at the indicated times post-infection. Infection was measured by luciferase assays. Relative inhibition (%) was determined by comparison with the control. qPCR analysis of (B) the formation of proviral DNA at reverse transcription, and (C) the integration of the proviral DNA in the host cell genome after compound treatment as indicated. NI: not infected. Data represent the means of quadruplicates with standard deviations shown as error bars.