Figure 3
From: Primate lentiviral Nef proteins deregulate T-cell development by multiple mechanisms
SF2 Nef proteins mutated in F195 show reduced PAK2 binding. In vitro kinase assay of AU1-tag immunoprecipitated Nef from cells transfected with SF2 Nef, constitutive active CDC42, and HA-tagged PAK2, as indicated. Upper panel shows Nef co-immunoprecipitated PAK2 activity, lower panel anti-AU1 staining of cell lysates on Western blot (numbers indicate densitometric quantification).