Figure 4

Reduced entry using GPR15 and CXCR6 by CD4-independent Envs. Env-pseudotyped viruses were tested for their ability to infect cells transfected with rmCD4 and either rmGPR15 or rmCXCR6. (A,B) Entry into 293 T cells transfected with rmCD4 and either rmGPR15 or rmCXCR6 was measured for each Env, and is indicated as a percentage of entry by that Env using rmCCR5 and rmCD4. (C,D) Envs from (A) or (B) were plotted showing entry in cells transfected rmCCR5 without CD4 on the X axis, and entry into cells transfected with rmCD4 and either rmGPR15 (C) or rmCXCR6 (D) on the Y axis. Each Env is indicated as a single point on the plot, and linear regression analysis was performed to assess the relationship between CD4-independent entry and GPR15 or CXCR6 use.