Figure 3
From: Contribution of oligomerization to the anti-HIV-1 properties of SAMHD1
Analysis of SAMHD1 oligomerization by size-exclusion chromatography and analytical ultracentrifugation. (A) Size exclusion chromatograms of the wild type (WT) and Y146S/Y154S 109–626 SAMHD1 constructs before and after incubation with dGTPαS. (B-E) Comparison plots of the diffusion-corrected integral sedimentation coefficient distributions obtained from a van Holde – Weischet analysis. WT and Y146S/Y154S without dGTPαS (B); WT incubated with 0, 25 and 50 μM dGTPαS (C); Y146S/Y154S incubated with 0, 25 and 50 μM dGTPαS (D); comparison of WT and Y146S/Y154S distributions following incubation with dGTPαS (E). Similar results were obtained in three independent experiments and a representative experiment is shown.