IgG1 and IgG2a antibody levels to SIVgp130 after cell transfer and subsequent VLP immunization. Donor mice (D) were immunized with adenoviral vectors encoding SIV GagPol or GFP. Six weeks later splenocytes (S), CD8-depleted splenocytes (4+), and CD4-depleted splenocytes (8+) were transferred into syngeneic recipient mice. The recipient mice and remaining donor mice were immunized with SIV VLPs 5 and 55 days after cell transfer. Mean and standard errors of IgG1 and IgG2a antibody levels to SIVgp130 of four animals per group are shown at the indicated days after the first VLP immunization. Statistically significant differences between the Ad-GFP and the Ad-Sgp group are marked by asterisks (p < 0.05, Mann Whitney test).