Figure 2
Co-culture model. (A) Schematic representation of model. Drug-exposed human explant tissue is washed, minced and co-cultured with HIV-susceptible cells (JT-CCR5 cells) for 72 h to allow drug present in the tissue to be transported into cells prior to challenge with HIV-1Ba-L. (B) Ectocervical explants were exposed to indicated concentrations of IQP-0528 for 24 hours. Tissue was washed and either immediately co-cultured with JT-CCR5 cells for 72 h prior to challenge or stored at -80C for 24 h or 7 d before co-culture and challenge. HIV replication was assessed by measuring p24 antigen in culture supernatants 7 days post-infection. Data are presented as the percentage infection relative to co-cultures challenged in the absence of drug treatment and are means ± SEM obtained from at least two independent experiments using tissue from different donors where each condition was tested in triplicate.