Figure 2

90K-induced reduction of HIV-1 particle infectivity correlates with defective gp120 incorporation into progeny, without influencing virus release. (A) Correlative analysis of gp120Env content per p24CA and HIV-1 particle infectivity (infectivity per ng p24) in the supernatant as a function of 90K plasmid amount. Small symbols represent the values of each individual transfection; large symbols represent the arithmetic means ± S.E.M. of five-six individual experiments. The Pearson’s correlation coefficient r and the corresponding p value were calculated using GraphPad Prism Software. (B) Correlative analysis of relative released virus and HIV-1 particle infectivity (infectivity per ng p24) in the supernatant as a function of 90K plasmid amount. Small symbols represent the values of each individual transfection; large symbols represent the arithmetic means ± S.E.M. of four individual experiments. The Pearson’s correlation coefficient r and the corresponding p value were calculated using GraphPad Prism Software. (C) Relative percentage of cell-associated gp120Env or gp160 Env versus total detected anti-gp120 signal as a function of cotransfected 90K plasmid amount. Values were obtained by Infrared imaging-based quantification of signals. Small symbols represent the values of each individual experiment; large symbols represent the arithmetic means ± S.E.M. of four individual experiments.