Figure 3

Semiquantitative RT-PCR of CHP, JUN-D and NFATc in controls and p30^II expressing Jurkat T lymphocytes (A and B) and primary CD4+ T lymphocyte (C and D) samples. PCR products were separated by electrophoresis (A and C), normalized to GAPDH and quantified by densitometry (B and D). In panel B, dark grey bars indicate indicate p30^II expressing cells and light grey bars indicate control (empty vector) cells. In panel D, dark grey bars indicate indicate p30^II expressing cells and white bars indicate control (empty vector) cells. Data points are mean of triplicates. CHP was downregulated while JUN-D and NFATc was upregulated by p30^II. Fold decrease/increase in activity in the presence of p30^II are indicated above each bar.