Triplicate p30II samples express GFP and p30II while triplicate controls express only GFP. (A) Flow cytometric analysis illustrating the expression of GFP in Jurkat T cells 10 days post spin-infection with lentiviral vectors. Both sample (expressing p30II and GFP) and control (GFP alone) group contains relatively high and similar levels of GFP. (B) RT-PCR demonstrating the expression of p30II in Jurkat T cells 10 days post spin-infection with lentiviral vectors. Jurkat T cells spin-infected with sample vector express p30II while the control vector spin-infected cells do not express p30II. RT-PCR was performed with triplicate samples and controls. GAPDH was used as a control for the integrity of the message. (C) Representative western blot showing p30II expression from cell lysate (p30II migrates at ~28 kD). M = Mock vector infected cell lysate, p30 lv = p30 lentivirus vector infected cell lystate, MW = biotin molecular weight markers.