Skip to main content

Advertisement

Figure 2 | Retrovirology

Figure 2

From: Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells

Figure 2

Impaired Th1 response induced by LV-transduced DCs. We analyzed T helper function by using DC:T cell co-culture and IL-4 and IFN-γICCS. Immature DCs were infected with mock (293T supernatants) or LV on d5 and treated with LPS and TNFα. The DCs were harvested and co-cultured with naïve CD4+ T cells at a DC:T cell ratio of 1:10. On Day 7 amd 9 after co-culture, the cells were re-stimulated and the T helper cell populations were examined by INF-γ and IL-4 antibody ICCS as described in the Materials and Methods. The percentages of cell populations are indicated in the FACS quadrants. The results are representative of four independent experiments.

Back to article page