Skip to main content
Figure 7 | Retrovirology

Figure 7

From: Specific TATAA and bZIP requirements suggest that HTLV-I Tax has transcriptional activity subsequent to the assembly of an initiation complex

Figure 7

Tax further activates a promoter with DNA-tethered TBP. (A) CAT reporter plasmid. Each plasmid contains two copies of 21-bp repeat, five copies of Gal4-binding sites and one copy of minimal promoter (TATAA) from adenovirus HTLV-I, HIV-1, SV40 and adenovirus E1b. (B) CAT assay. HeLa cells were co-transfected with 5 μg of the indicated CAT reporter plasmids (p21-G5-HTLV-CAT, p21-G5-HIV-CAT, p21-G5-SV40-CAT and p21-G5-E1B-CAT) and 2 μg of pGal4-TBP (yellow) or 2 μg of pIEX (Tax; pink) or 2 μg of pGal4-TBP plus 2 μg of pIEX (Gal4-TBP + Tax; blue). Basal CAT activity from cells transfected with pGal4-TBP plus p21-G5-E1B-CAT was taken as 100% (group 4, yellow).

Back to article page

Retrovirology

ISSN: 1742-4690

Contact us