Trans-dominant cellular inhibition of DC-SIGN-mediated HIV-1 transmission

Retrovirology

Table 2 Expression of HIV-1 receptors and adhesion molecules on donor cells.^a

Surface ligands	Dendritic cells^b	Raji/ DC-SIGN	K562/ DC-SIGN
CD4	+	-	-
CCR5	+	-	-
CXCR4	+	+	+
Duffy antigen/receptor for chemokines (DARC)	ND	-	+/-
HLA-I (MHC-I)	++	++	+
HLA-DR (MHC-II)^c	++	++	-
ICAM-1 (CD54)	+	+	+
ICAM-2 (CD102)	+	+	+
ICAM-3 (CD50)	+	-	-
LFA-1 (CD11a/CD18)	+	+	-
LFA-3 (CD58)	+	+	+
B7-1 (CD80)	+	+	+
B7-2 (CD86)	+	+	+

^a Proteins expressed on the surface of the donor cells were stained and analyzed by FACS. Isotype-matched mouse IgG controls were also examined. Cell population expression levels indicated as ++, +, and +/- demonstrated a mean fluorescence of antibody staining greater than 50, 10, and 7.5, respectively. No antibody and isotype control stainings typically displayed a mean fluorescence intensity of 5 in the FL-2 channel, using a Becton-Dickinson FACSCalibur instrument. ND, not done. Staining of parental Raji and K562 cells was uniformly negative for DC-SIGN and showed a similar expression pattern of these ligands (data not shown). ^b Immature monocyte-derived dendritic cells were stained at day 7 of culture in the presence of cytokines [5]. ^c HLA-DP and -DQ expression was also examined and results were similar to what is shown for HLA-DR.

ISSN: 1742-4690