Figure 2

Specificity of DNA hybridizations. Each bar represents the hybridization results for each gene. These were calculated as the mean intensity using infected host cell DNA and viral DNA versus uninfected host cell DNA. The color of the bar is a function of the ratio and indicative of Cy3 hybridization intensity for virus DNA relative to the hybridization (Cy5) intensity for uninfected host cell DNA. Spotted probes are PCR amplicons of the viral and human control genes and are indicated below each graph. The labeled targets are indicated to the left. (A) Hybridization of genomic DNA isolated from virally infected cells. (B) Hybridization of plasmid DNA labeled by nick translation. (C) Hybridization of genomic DNA isolated from dually (KSHV and EBV) infected cells, Cra-BCBL and BBG1. Hybridizations that resulted in a ratio of less than five for (A&C) or less than two for (B) were considered as non-hybridization and are indicated in blue. Hybridizations that resulted in a ratio of five to ten for (A&C) or two to five for (B) were considered low hybridization intensities and are indicated in yellow, while high hybridizations resulting in a ratio greater than ten for (A&C) or five for (B) are indicated in red.