RTE and CTE mRNA export elements synergistically increase expression of unstable, Rev-dependent HIV and SIV mRNAs
© Smulevitch et al; licensee BioMed Central Ltd. 2006
Received: 07 November 2005
Accepted: 13 January 2006
Published: 13 January 2006
Studies of retroviral mRNA export identified two distinct RNA export elements utilizing conserved eukaryotic mRNA export mechanism(s), namely the Constitutive Transport Element (CTE) and the RNA Transport Element (RTE). Although RTE and CTE are potent in nucleocytoplasmic mRNA transport and expression, neither element is as powerful as the Rev-RRE posttranscriptional control. Here, we found that whereas CTE and the up-regulatory mutant RTEm26 alone increase expression from a subgenomic gag and env clones, the combination of these elements led to a several hundred-fold, synergistic increase. The use of the RTEm26-CTE combination is a simple way to increase expression of poorly expressed retroviral genes to levels otherwise only achieved via more cumbersome RNA optimization. The potent RTEm26-CTE element could be useful in lentiviral gene therapy vectors, DNA-based vaccine vectors, and gene transfer studies of other poorly expressed genes.
Posttranscriptional events determine the fate of cellular and viral mRNAs through concerted actions promoting nuclear trafficking and cytoplasmic transport, stabilization and translation. Simian type D (SRV/D) retroviruses and intracisternal A-particle retroelements (IAP) have provided us with unique mRNA transport elements, which utilize conserved cellular export machinery [1–13]. The export of the SRV/D unspliced mRNA is mediated by the cis-acting constitutive transport element CTE [8, 10–13] through interaction with the cellular NXF1 protein , which is also the key factor mediating general mRNA export [1–5], a property which is conserved among eukaryotes (reviewed in [14–16]). We previously identified another functionally similar but structurally unrelated posttranscriptional RNA Transport Element RTE [6, 7], which is present in a subgroup of murine IAP. Both CTE and RTE utilize the conserved eukaryotic mRNA transport machinery. Here, we demonstrate that the combination of RTE and CTE in cis leads to synergistic increase in lentiviral gene expression.
Synergistic activation of gene expression in the presence of a combination of RTE-CTE
Synergistic effect of RTEm26CTE on HIV-1 env expression
To rule out that the observed synergistic effect is a unique feature of the gag reporter mRNA, we inserted RTEm26-CTE into an HIV-1 env reporter plasmid pNL1.5E (Figure 2A), expressing the authentic env cDNA from the HIV-1 LTR promoter. Like gag, env is poorly expressed (Figure 2B, lane 8) in the absence of a positive-acting export system, as expected. Both plasmids, containing either CTE (lanes 1, 2) or RTE (lanes 3, 4) alone, showed ~10× fold increase in Env production compared to the pNL1.5E (lane 8). The presence of RTEm26-CTE led to an additional increase in Env production (lane 5). A semi-quantitative analysis using serial dilutions (lanes 5–7) of the cell extract shows a ~100× fold activation, confirming synergistic effect of RTEm26-CTE. This expression level was comparable to that obtained in the presence of Rev (lanes 9–11). These data demonstrate that the synergistic effect of the combination of RTEm26-CTE export elements is applicable for different poorly expressed, unstable HIV-1 mRNAs.
Synergistic effect of RTEm26CTE on expression of a Rev- and RRE-deficient HIV-1 and SIV molecular clones
Upon insertion of CTE or RTE alone into the Rev- and RRE-minus NL4-3, we had previously shown that these RNA transport elements are able to partially replace the viral Rev-RRE system resulting in the production of infectious virus [6, 9, 11, 12] (Figure 3B and 3C). Interestingly, Western immunoblot analysis showed that the presence of RTEm26-CTE mediated a dramatic synergistic increase in expression of both env and gag compared to the clones containing each element alone (Figure 3B). Quantitation of gag expression using an antigen capture assay showed an increase of ~1 log over the presence of CTE or RTE alone. The expression level in the presence of RTEm26-CTE was only slightly lower (~3x-fold) than those obtained by the wild type HIV-1 NL4-3 (Figure 3B). Upon infection of Jurkat cells, the RTEm26-CTE containing Rev-independent HIV-1 clone as well as the RTE- or CTE-containing clones showed similar replicative capacities to levels ~1 log lower than that of the wild type HIV-1 (Figure 3C). Thus, the presence of RTEm26-CTE is able to promote a balanced expression of the viral proteins able to generate infectious virus.
Similarly, we found that the presence of RTEm26-CTE also greatly increased expression of the Rev- and RRE-minus molecular clone of SIVmac239 (Figure 4B) to levels about ~10x-fold higher than those obtained by SIV clone containing only the CTE. Like its HIV counterpart, the RTEm26-CTE-containing SIV produces infectious virus (Figure 4C). We noted that it replicates with growth kinetics similar to the wild type SIV, in both CEMx174 cells (Figure 4C) and monkey PBMCs (data not shown), despite its slightly reduced level of expression (Figure 4B). In contrast to its HIV counterpart, the presence of the more potent RNA export element combination improved the replicative capacity when compared to the virus that contains only the CTE (compare peak at day 14 postinfection). Since we could not test propagation of SIV and HIV in the same cell types, it is possible that cellular factors may contribute to this phenomenon and this was not further investigated.
In conclusion, we have shown that the potent posttranscriptional effect of the RTEm26-CTE combination of RNA export elements from simple expression vectors (Figures 1 and 2) as well as from the complex array of mRNAs produced from the molecular clones of HIV and SIV (Figures 3 and 4).
Synergy depends on the spatial arrangement of RTEm26 and CTE
We then tested whether the spatial arrangement of RTEm26 and CTE contributed to the synergistic effect. First, the reversal of the order of the elements from RTEm26-CTE to CTE-RTEm26 (Figure 5B) showed that the combination of the elements functions similarly in either configuration. Second, the 28 nt spacer between the elements was increased by insertion of a 325-nt spacer sequence (SP1), which led to a significant loss of synergy (Figure 5B). To exclude that the nature of the spacer RNA contributed to this effect, a different RNA fragment (SP2) was used (Figure 5B), resulting in a similar decrease in gag expression. Separation of the elements by shorter spacers of 202 and 100 nt led to gradual decrease in RTEm26-CTE activity (data not shown). Thus, the optimal synergistic effect requires the up-regulatory mutant RTE (RTEm26) and a functional CTE at close proximity.
The question arose whether multimers of CTE or RTE alone could achieve a similar effect. The presence of a CTE multimer has been reported to improve expression i.e. 4xCTE in a gag/pol reporter further elevated expression but this depended on the nature of the polyA signal , whereas multiple copies of the CTE had little or no effect in other mRNAs (, our own observations), suggesting that an effect of CTE multimers depended on the mRNA context. Using the gag reporter plasmid used herein, pNLgag, we found that two adjacent CTE elements also synergized reaching ~75% of the effect observed for RTEm26-CTE. In contrast, we found that RTEm26 does not synergize with itself. Thus, while the effect of CTE multimers is transcript dependent, the RTEm26-CTE mediated synergistic increase in gene expression was persistently observed using different mRNAs (Figures 1, 2, 3, 4). Most importantly, the use of RTEm26-CTE has another great advantage, because this combination avoids the presence of adjacent repeated sequences, which may cause plasmid instability during growth in bacteria.
The rather unexpected finding of this work was that the combination of two retroviral/retroelement-derived cis-acting RNA export elements, RTE and CTE, synergistically increased expression of different retroviral mRNAs that are otherwise poorly expressed (Figures 1, 2, 3, 4). Since the function of RTEm26-CTE is conserved in mammalian cells, their integration into expression vectors provides a potent tool to improve expression of poorly expressed, unstable retroviral mRNAs to levels otherwise only achieved via more cumbersome RNA optimization.
Whereas the main restriction retroviral mRNAs encounter is their nucleocytoplasmic transport, other mRNAs may have different restrictions. The question arises whether the RTEm26-CTE combination has any effect on the expression of genes or cDNAs, which have posttranscriptional restrictions other than those of the lentiviral mRNAs. No improvement of expression was found using either the RTE, the CTE or the RTEm26-CTE combination in a MuLV-derived retroviral vector , while insertion of the woodchuck element WPRE augmented expression of this MuLV mRNA. These data indicate that oncoretrovirus and lentivirus expression have distinct restrictions. We further tested whether the presence of these RNA export elements could counteract posttranscriptional control that is not exerted at the RNA transport level but only involves cytoplasmic control. We noted that these export elements, as expected, did not alleviate the downregulatory effect of the AU-rich element (ARE)-containing IL-3 mRNA using the GFP-IL-3 hybrid mRNAs as a model system . Thus, this finding supports the specific mechanism of function of the RTE, CTE and RTEm26-CTE, namely nucleocytoplasmic export. For this reason, we tested RTEm26-CTE effect upon insertion into some of our already RNA-optimized HIV and SIV gag and env cDNAs vectors, whose mRNAs are efficiently exported leading to very high expression in cultured cells. As expected, we only found a less than 2-fold effect on this already optimized mRNAs. It remains to be tested whether export signals like the RTEm26-CTE could act as additional positive acting signals and mediate higher expression levels in primary cells, for example upon DNA vaccination of animals or using gene transfer vectors.
NXF1 provides a key molecular link between mRNA and components of the nuclear pore complex. A possible model to explain the synergistic effect of RTE and CTE is that the duplication of these export elements may provide an improved target for NXF1 resulting in more efficient nucleocytoplasmic mRNA transport. Using in vitro gel-shift assays, we found that the binding of NXF1 (aa 61–372) to radiolabeled CTE is competed similarly by both excess cold CTE as well as RTEm26-CTE RNAs (data not shown). These data indicate that NXF1 binds to CTE as well as to the RTEm26-CTE RNA targets with similar affinity. We have previously shown that NXF1 is not a high affinity binder of RTE when compared to the CTE , suggesting the role of a distinct cellular protein mediating RTE RNA export. It is plausible that this putative factor tethers the RTE-RNAs directly or indirectly to the NXF1 pathway. Therefore, it is likely that the putative RTE-binding protein and NXF1 may act cooperatively. Studies are on-going to delineate the detailed mechanism of function mediating this cooperativity.
mRNA expression is controlled at several steps including nuclear export, cytoplasmic trafficking and polysomal association. The use of strong mRNA export elements is a powerful tool to alleviate restrictions linked to nuclear export. For a subset of lentiviral mRNAs encoding gag, pol or env, posttranscriptional control has been shown at multiple steps of export and polysomal association. The presence of potent RNA export elements is sufficient to alleviate all of theses restrictions. Integration of RTEm26-CTE into lentiviral vectors will increase gene expression essential for applications such as in gene therapy that are otherwise only obtained through RNA optimization. For DNA-based vaccine vectors, it remains to be seen whether the presence of strong binding sites for the cellular mRNA transport machinery is of further advantage when introduced into primary tissues in animals as compared to cultured cells. In addition, these retroviral/retroelement derived RNA export elements provide unique tools to further dissect mechanisms involved in posttranscriptional regulation of viral and cellular genes.
The use of the combination of RNA export elements, comprising the up-regulatory mutant RTEm26 and the CTE, potently increase lentiviral gene expression.
The RTE, RTEm26, and CTE were inserted into pNLgag [23, 24, 39] between the gag gene and the 3'LTR and have been described . RTE or RTEm26 was inserted into the SacII site located 5' to the CTE, generating pNLgagRTE-CTE and pNLgagRTEm26-CTE, respectively. In pNLgag RTEm26-SP1-CTE, a spacer sequence (SP1) of 325 nt from a synthetic HIV-1 tat gene (BamHI-XbaI from plasmid 32H) was inserted between RTEm26 and CTE. In pNLgag RTEm26-SP2-CTE, a spacer (SP2) from a different source (cat gene) of 325 nt was inserted. Similarly spacers or 202 and 100 nt were inserted. The bovine growth hormone polyadenylation signal was inserted between SalI and XhoI sites 3' to RTEm26-CTE replacing the 3'LTR. pNLcgag  is similar to pNLgag, except it lacks the major splice donor. pNL1.5E expresses the authentic HIV-1 env cDNA from the LTR promoter . RTE, CTE and RTEm26-CTE were inserted as SmaI-XhoI fragment between the env gene and the 3' LTR into BlpI and XhoI digested pNL1.5E. The Rev-independent clones of NL4-3 [RRE(-)Rev(-), RRE(-)Rev(-)CTE, and RRE(-)Rev(-)RTE] have been published previously [6, 12, 41]. RTEm26-CTE was inserted into the XhoI site of the RRE(-)Rev(-) NL4-3. The SIVmac239 RRE(-)Rev(-)nefdelCTE is similar to the published SIVmac239 RRE(-)Rev(-)Nef(-)CTE  but contains an additional deletion of the remaining nef region 3' to the CTE . RTEm26-CTE was inserted in the place of CTE. The GFP-IL-3 plasmid contains the IL3 3'UTR inserted 3' to the enhanced green fluorescent protein (GFP) gene in pFRED25 . RTEm26, CTE, or RTEM26CTE were inserted between GFP and the 3'UTR. These elements were further inserted between the cDNAs and the polyadenylation signals of vectors expressing the RNA-optimized HIV-1 env (75 H).
Human HLtat, a HeLa-derivative producing HIV tat  or human 293 cells were transfected with 1 μg of the NLgag plasmids. HLtat provides Tat to activate gene expression from the viral LTR promoter. For transfection of 293 cells a tat expression plasmid, pBstat, was also co-transfected. We routinely analyzed 2–3 independent clones in duplicate determinations. Two to three days later, the cell extracts were analyzed for Gag expression using a commercial HIV-1 p24gag or the SIV p27gag antigen capture assay. Gag and Env production was also analyzed on Western immunoblot using plasma from HIV-1 infected persons, rabbit anti-HIV-1 env serum or SIVmac infected rhesus macaques . Cotransfection of 0.8 μg of the GFP expression vector pFRED25  served as internal control. Cotransfection of the secreted version of alkaline phosphatase SEAP  as internal control was used in some experiments and SEAP levels were determined from the culture supernatant using a commercial kit (Tropix, Inc.). Transfections of 293 cells were performed using FUGENE-6, whereas the Calcium-phosphate coprecipitation technique was used for HeLa cells. GFP-IL3 plasmids were transfected into NIH3T3 cells and analyzed by fluorescent activated cell sorting (FACS) as described . Total and cytoplasmic polyadenylated mRNA was isolated and analyzed as described [12, 46]. Hybridization of the blots with a GFP probe was used to evaluate transfection and RNA extraction efficiency. Blots were quantitated using the STORM860 phosphoimager.
constitutive Transport Element
RNA Transport Element
human immunodeficiency virus type 1
simian immunodeficiency virus
intracisternal A-particle retroelement
simian type D retroviruses
nuclear export factor 1.
We thank S. Lindtner for comments, our Werner H. Kirsten Student Intern program recipients C. Jodrie, A. Gainer, L. Kotani, T. Hudzik, and S. Sadtler for their contributions, L. Arthur and J. Lifson for antiserum, M. Lu for technical assistance, and T. Jones for editorial assistance. AvG and MR are contractors through SAIC. This research was supported by the Intramural Research Program of the National Institutes of Health, National Cancer Institute.
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