Figure 6
From: N-terminal Slit2 inhibits HIV-1 replication by regulating the actin cytoskeleton
Slit2N inhibits HIV-1-envelope-mediated actin polymerization. (A) MT4 cells were pre-treated with Slit2N (3nM) and treated with gp120 for various time points, as indicated. Cells were taken at various time points and stained with FITC-phalloidin and quantified by flow cytometry. The graph represents the percentage increase in F-actin positive cells over the control. (B) The cells stained with phalloidin-FITC were also observed under a fluorescence microscope. The images shown are cells stimulated with Gp120 for 5 minutes. (C) Resting T-cells were pre-treated with Slit2N (3nM) and treated with gp120 for various time points, as indicated. Cells were taken at various time points and stained with FITC-phalloidin and quantified by flow cytometry. The graph represents the percentage increase in F-actin positive cells over the control. (D) The cells stained with phalloidin-FITC were also observed under a fluorescence microscope. The images shown are cells stimulated with Gp120 for 5 minutes.