Figure 4
From: N-terminal Slit2 inhibits HIV-1 replication by regulating the actin cytoskeleton
Slit2N does not affect HIV-1 integration or transcription. (A) MT4 cells were treated with Slit2N prior to HIV-1 infection or three hours after infection (to bypass the entry step). Twenty-four hours after infection, DNA was extracted from the respective samples, and the integrated HIV-1 DNA was analyzed by qPCR for Alu-LTR. (B) The full length and spliced (C) RNA transcription was evaluated by qRT-PCR. Uninfected and HIV-1 infected MT4 cells were used as controls. Results are representative of 3 separate experiments.