Figure 2

Slit2N inhibits HIV infection of activated and resting CD4⁺T-cells. (A) PHA-stimulated CD4⁺ T-cells were infected with HIV-1 IIIB (left panel) or HIV-1 BaL (right panel) in the presence of Slit2N (3nM). The cells were incubated in IL-2 containing medium, and the cells were collected after 48 hours and analyzed for HIV-1 p24 expression by flow cytometry. *P < 0.05 versus untreated cells infected with HIV-1. (B) Resting CD4⁺ T-cells were infected with HIV-1 IIIB in the presence or absence of Slit2N (3nM). Following infection, the cells were washed to remove unbound virus and cultured in the presence of Slit2N for 5 days. Cells were activated at day 5 with anti-CD3/CD28 magnetic beads, and viral replication was measured by p24 release. Results are representative of 3 separate experiments.