Fig. 5
From: Attenuation of reverse transcriptase facilitates SAMHD1 restriction of HIV-1 in cycling cells
The effect of high and low SAMHD1 expression levels. A Plot of flow cytometry data indicating the populations of WT-SAMHD1 transduced U937 cells sorted as high (SAMHD1Hi), medium (SAMHD1Me) and low (SAMHD1Lo) expressing populations. B–C Restriction of HIV-1-GFP RT-Q151N by SAMHD1Hi (solid circles) and SAMHD1Low (open circles) cells expressing the indicated SAMHD1 proteins (WT, T592C, T592D, HD206-7AA and R164A) in B cycling and C differentiated U937 cells. The infectivity ratio was calculated as the percentage of GFP positive cells in the YFP (SAMHD1) positive population divided by the percentage of GFP positive cells in the YFP (SAMHD1) negative population. D Cell dNTP levels measured from 106 sorted cycling (left) and differentiated (right) U937 cells with SAMHD1Hi, SAMHD1Me, and SAMHD1Lo expression. The amounts of dATP, TTP, dCTP, and dGTP within the total bar stack are shown in blue, yellow, beige, and red respectively. The values are the mean of two technical repeats and error bars represent the SD