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Fig. 5 | Retrovirology

Fig. 5

From: HIV-1 subtype C Nef-mediated SERINC5 down-regulation significantly contributes to overall Nef activity

Fig. 5

SERINC5 down-regulation activity of Nef mutants. A Flow cytometry plots of the consensus C Nef (WT) and the consensus C Nef 173T mutant (173T) are shown, with SERINC5 cell-surface expression on the y axis and the green fluorescent protein (GFP) expressing cells (representing cells transfected with Nef clones) on the x axis. The SERINC5 down-regulation activities of the consensus C Nef mutants (B) and SK446 Nef mutant (C) normalised to the respective WT proteins (representing 100% activity) are shown, where the data represents the means and standard deviations of three independent experiments. The SERINC5 down-regulation ability expressed relative to SF2 Nef was 80% and 91% for wild-type (WT) consensus C Nef and SK446 Nef, respectively. ANOVA with Tukey post-hoc tests was performed to assess which consensus C Nef mutants differed significantly from WT, and the Student’s T test was used to compare the down-regulation activity of the SK446 WT and SK446 173T mutant. The ANOVA/Student’s T test p values are shown and the Tukey post hoc test p value is indicated by asterisks (*** is p < 0.001)

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