Fig. 1

SERINC5 down-regulation activities of patient-derived Nef clones. A Representative flow cytometry plots of the positive control (SF2 Nef) and negative control (SF2 Nef with the G2A mutation, rendering it inactive for SERINC5 down-regulation) are shown. The median fluorescence intensity (MFI) values indicate SERINC5 cell-surface expression in green fluorescent protein (GFP) expressing cells (representing cells transfected with Nef clones). B The SERINC5 down-regulation activities of Nef clones derived from patients from the HPP acute infection, TRAPS and Tshedimoso cohorts are shown. SERINC5 down-regulation activities of Nef clones were expressed relative to SF2 (100% down-regulation) and G2A (0% down-regulation)