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Fig. 4 | Retrovirology

Fig. 4

From: HUSH-mediated HIV silencing is independent of TASOR phosphorylation on threonine 819

Fig. 4

Mutation of TASOR T819 does not impact TASOR repressive effect. HeLa HIV-1 LTR-ΔTAR-Luc were TASOR-depleted by CRISPR/Cas9. These TASOR depleted cells were then transfected with vectors encoding for HA-TASOR, HA-TASOR TA, HA-TASOR TE or HA-TASOR ΔPARP. Luc activity was measured, normalized on BCA, and proteins were analyzed by Western-blot 48 h post-transfection (n = 3; A two-sided unpaired t-test was used)

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