Fig. 2
From: HUSH-mediated HIV silencing is independent of TASOR phosphorylation on threonine 819
TASOR is phosphorylated by a Cyclin/CDK complex on T819. A Hela cells were treated or not with nocodazole to induce TASOR phosphorylation, and with various concentrations of the Cyclin/CDK roscovitine inhibitor. B CDK1 and CDK2 expression was silenced using siRNA, then cells were treated or not with nocodazole and the subsequent phosphorylation of TASOR was analyzed by western blot together with the expression of the indicated proteins. C Following overexpression of TASOR-Flag or SAMHD1-Flag by transfection, an anti-Flag immunoprecipitation was carried out to test a potential interaction of these proteins with CDK1 and CDK2