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Fig. 4 | Retrovirology

Fig. 4

From: Visualization of clonal expansion after massive depletion of cells carrying the bovine leukemia virus (BLV) integration sites during the course of disease progression in a BLV naturally-infected cow: a case report

Fig. 4

Visualization of the integration site identified on Chr 1. A Visualization of NGS reads detected on IGV profile. B Schematic diagram to detect both Chr 1/BLV-5′LTR chimeric fragment and BLV-3′LTR/Chr 1 chimeric fragment. Arrows indicate the position of primers. C Confirmation of the Chr 1/BLV-5′LTR chimeric fragment using conventional PCR and Sanger sequence. Arrow indicates the PCR product of the Chr 1/BLV-5′LTR chimeric read. M, 100 bp DNA Ladder marker (MIXELL Inc, Tokyo, Japan); 1, Stage I; 2, Stage II; 3, Stage III; C, no-template DNA-negative control (water substituted for DNA template). Six nucleotides duplication of host sequence (GTAAAA) was detected by Sanger sequence. D Confirmation of the BLV-3′LTR/Chr 1 chimeric fragment using conventional PCR and Sanger sequence. Arrow indicates the PCR product of the BLV-3′LTR/Chr 1 chimeric fragment. M, 100 bp DNA Ladder marker (MIXELL Inc, Tokyo, Japan); 1, Stage I; 2, Stage II; 3, Stage III; C, no-template DNA-negative control (water substituted for DNA template). 6 nucleotides duplication of host sequence (GTAAAA) colored in red was detected by Sanger sequence

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Retrovirology

ISSN: 1742-4690

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