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Fig. 5 | Retrovirology

Fig. 5

From: Replication-competent HIV-1 in human alveolar macrophages and monocytes despite nucleotide pools with elevated dUTP

Fig. 5

Viruses collected from QVOA supernatants of AM, MC and T cells can establish de novo infection. A Supernatants from QVOA positive wells were used to separately spin infect 200,000 MOLT4/CCR5 cells using serial dilutions. Supernatants were collected at days 0, 4, 10, and 15 after spin infection and HIV RNA copies were determined using RT-qPCR. B Reinfection kinetics of AM and T cells obtained from P2 QVOA supernatant. C Reinfection kinetics of MC and T cells obtained from P3 QVOA supernatant. D Reinfection kinetics of MC and T cells obtained from P4 QVOA supernatant. E Reinfection kinetics of MC and T cells obtained from P5 QVOA supernatant. F Percentage of replicating viral particles in HIV RNA copies detected in QVOA. HIV RNA copies in the culture supernatants were measured by RT-qPCR and the frequency of replicating viral particles was calculated using an Infection Frequency Calculator that utilizes limiting dilution statistics (https://silicianolab.johnshopkins.edu). The replicating virus percentage of AM, MC, and T cells for each patient assayed is shown. The data in B, C, D, and E show the mean value of three technical replicates. The RNA copies for each patient in panel F were measured in three replicates. The error bars are smaller than the data points in the graph

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