Fig. 2
Comparison of in vitro infection of AM and MDM with HIVBal. A Kinetics for appearance of early (ERT) and late (LRT) reverse transcripts in AM and MDM as determined by RT-qPCR. The RPP30 gene was used to calculate the average transcript copies per cell. Sequences and probes are listed in Additional file 1: Table S1. B Kinetics for appearance of proviral DNA copies in MDM and AM as determined by alu-gag qPCR. C Percentage of proviruses present in MDM and AM that contain dUMP as determined by alu-gag Ex-qPCR. D Viral protein 24 (p24) levels in culture supernatants of infected AM and MDM were measured by ELISA. The supernatant p24 levels are from identical infections (MOI = 1) using equal numbers of plated target cells (50,000 per well in 96-well plate). Errors are means with standard deviations, N = 2 for A–C. For panel D, N = 1Â